SMO anticorps (Internal Region)

N° du produit ABIN7540600

N° du produit (Fournisseur): 600-401-mg0s

Cet anticorps anti-SMO est un anticorps Lapin Polyclonal détectant SMO dans WB, ELISA et IHC. Adapté pour Humain.

Aperçu rapide pour SMO anticorps (Internal Region) (ABIN7540600)

Antigène: SMO (Smoothened Homolog (Drosophila) (SMO))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMO est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-SMO anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-mg0s

Fournisseur: Rockland

Fonction: Smoothened Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human SMO.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène: Anti-Smoothened antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human Smoothened conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1 μg/mL

Commentaires:

Anti-Smoothened Antibody has been tested in ELISA, WB, and IHC.  Expect a band at ~86.4kDa in western blot using appropriate lysates and tissues.  Positive control used: Overexpressing lysate and HEK293T in Western Blot, and Human Lung Tissue in Immunohistochemistry.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur SMO

Antigène: SMO (Smoothened Homolog (Drosophila) (SMO))

Autre désignation: SMO

Sujet: Rabbit Anti-SMO Antibody, Smoothened Frizzled Class Receptor,  Smoothened Seven Transmembrane Spanning Receptor, Smoothened Frizzled Family Receptor, Frizzled Family Member 11, Protein GX, SMOH, Seven Transmembrane Helix Receptor, Smoothened (Drosophila) Homolog, Smoothened Homolog (Drosophila), Smoothened Homolog, FZD11, CRJS, Gx,SMO (Smoothened, Frizzled Class Receptor) receptors are non-classical G protein-coupled receptors that probably associates with the patched protein (PTCH) to transduce the hedgehogs proteins signal. The binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). It is required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. And it interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation.  Diseases associated with SMO include Curry-Jones Syndrome and Basal Cell Carcinoma.  Anti-Smoothened Antibody is useful for researchers interested in Cancer research.

ID gène: 6608

UniProt: Q99835

Pathways: Signalisation Hedgehog